Skip to main content
Log in

Biodegradation of phenol from a synthetic aqueous system using acclimatized activated sludge

  • Original Paper
  • Published:
Arabian Journal of Geosciences Aims and scope Submit manuscript

Abstract

Phenol is one of the aromatic hydrocarbons. Phenol and its derivatives are highly toxic. These pollutants can be observed in the effluents of many industries. This research investigates the removal of phenol by the use of activated sludge in a batch system. The effects of influencing factors on biodegradation efficiency have been evaluated. The main factors considered in this study were the volume of acclimatized activated sludge inoculation, pH, temperature, and initial concentration of phenol. The inoculation volumes of 1, 3, and 5 mL of acclimatized activated sludge were taken into account. Different pH values of 3, 5, 7, 9, and 11 were examined. The experiments were conducted for temperatures of 25, 30, 35, and 40 °C and initial phenol concentrations of 400, 800, 1,000, and 1,500 ppm. The results show that the acclimatized activated sludge has a high capacity for the removal of phenol. From a 100-mL aqueous solution was removed 1,500 ppm of phenol after 80 h. Furthermore, maximum phenol removal was observed for an inoculation volume of 5 mL for three different phenol concentrations of 100, 400, and 800 ppm. The best pH was 7 for the biodegradation process, and the optimum temperature was 30 °C. It was further found that an increase in the phenol concentration increased its removal time. Moreover, the activated sludge could effectively remove about 99.9 % of phenol from a synthetic aqueous solution in a batch system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Ahmed AM, Nakhla GF, Farooq SJ (1995) Phenol degradation by Pseudomonas aeruginosa. J Environ Sci Health 30:99–107

    Google Scholar 

  • Aksu Z, Gönen F (2004) Biosorption of phenol by immobilized activated sludge in a continuous packed bed: prediction of breakthrough curves. Process Biochem 39:599–613

    Article  Google Scholar 

  • Alexievaa Z, Gerginova M, Zlateva P, Peneva N (2004) Comparison of growth kinetics and phenol metabolizing enzymes of Trichosporon cutaneum R57 and mutants with modified degradation abilities. Enzym Microbiol Technol 34:242–247

    Article  Google Scholar 

  • Arutchelvan V, Kanakasabai V, Elangovan R, Nagarajan S, Muralikrishnan V (2006) Kinetics of high strength phenol degradation using Bacillus brevis. J Hazard Mater 129:216–222

    Article  Google Scholar 

  • Bergauer P, Fonteyne PA, Nolard N, Schinner F, Margesin R (2005) Biodegradation of phenol and phenol-related compounds by psychrophilic and cold-tolerant alpine yeasts. Chemosphere 59:909–918

    Article  Google Scholar 

  • Berkowitz JB (1988) Standard handbook of hazardous water treatment and disposal—hazardous waste recovery processes. McGraw-Hill, New York

    Google Scholar 

  • Buitron G, Gonzalez A, Lopez-Marin LM (1998) Biodegradation of phenolic compounds by an acclimated activated sludge and isolated bacteria. Water Sci Technol 37:371–378

    Google Scholar 

  • Chedeville O, Debacq M, Almanza MF, Porte C (2007) Use of an ejector for phenol containing water treatment by ozonation. Sep Purif Technol 57:201–208

    Article  Google Scholar 

  • Contreras EM, Albertario ME, Bertola NC, Zaritzky NE (2008) Modelling phenol biodegradation by activated sludges evaluated through respirometric techniques. J Hazard Mater 158:366–374

    Article  Google Scholar 

  • Gorska P, Zaleska A, Hupka J (2009) Photodegradation of phenol by UV/TiO2 and Vis/N, C-TiO2 processes: comparative mechanistic and kinetic studies. Sep Purif Technol 68:90–96

    Article  Google Scholar 

  • Kumarl A, Kumar S, Kumar S (2005) Biodegradation kinetics of phenol and catechol using Pseudomonas putida MTCC 1194. Biochem Eng J 22:151–159

    Article  Google Scholar 

  • Lee S, Basu S, Tyler CW, Wei IW (2004) Ciliate populations as bio-indicators at Deer Island treatment plant. Adv Environ Res 8:371–378

    Article  Google Scholar 

  • Marrot B, Martinez AB, Moulin P, Roche N (2006) Biochemical biodegradation of high phenol concentration by activated sludge in an immersed membrane bioreactor. Biochem Eng J 30:174–183

    Article  Google Scholar 

  • Nemerow NL (1978) Industrial water pollution: origin, characteristics and treatment. Addison-Wesley, London

    Google Scholar 

  • Onysko KA, Budman HM, Robinson CW (2000) Effect of temperature on the inhibition kinetics of phenol biodegradation by Pseudomonas putida Q5. Biotechnol Bioeng 70:291–299

    Article  Google Scholar 

  • Patterson JW (1985) Industrial wastewater treatment technology, 2nd edn. Butterworths, USA

    Google Scholar 

  • Sá CSA, Boaventura RAR (2001) Biodegradation of phenol by Pseudomonas putida DSM 548 in a trickling bed reactor. Biochem Eng J 9:211–219

    Article  Google Scholar 

  • Salvado H, Gracia MP (1993) Determination of organic loading rate of activated sludge plants based on protozoan analysis. Water Res 27:891–895

    Article  Google Scholar 

  • Santos VL, Linardi VR (2004) Biodegradation of phenol by a filamentous fungi isolated from industrial effluents-identification and degradation potential. Process Biochem 39:1001–1006

    Article  Google Scholar 

  • Sittig M (1997) How to remove pollutants and toxic materials from air and water—a practical guide. Noyes Data Corporation, Park Ridge

    Google Scholar 

  • Tryba B, Morawski AW, Inagaki M, Toyoda M (2006) The kinetics of phenol decomposition under UV irradiation with and without H2O2 on TiO2, Fe–TiO2 and Fe–C–TiO2 photocatalysts. Appl Catal B: Environ 63:215–221

    Article  Google Scholar 

  • Tsai SY, Juang RS (2006) Biodegradation of phenol and sodium salicylate mixtures by suspended Pseudomonas putida CCRC 14365. J Hazard Mater 138:125–132

    Article  Google Scholar 

  • Wang SJ, Loh KC (2001) Biotransformation kinetics of Pseudomonas putida for cometabolism of phenol and 4-chlorophenol in the presence of sodium glutamate. Biodegrad 12:189–199

    Article  Google Scholar 

  • Wang G, Wen J, Yu G, Li H (2008) Anaerobic biodegradation of phenol by Candida albicans PDY-07 in the presence of 4-chlorophenol. World J Microbiol Biotechnol 24:2685–2691

    Article  Google Scholar 

  • Wang P, Qu Y, Zhou J (2009) Biodegradation of mixed phenolic compounds under high salt conditions and salinity fluctuations by Arthrobacter sp. W1. Appl Biochem Biotechnol 159:623–633

    Article  Google Scholar 

  • Ying C, Umetsu K, Ihara I, Sakai Y, Yamashiro T (2010) Simultaneous removal of organic matter and nitrogen from milking parlor wastewater by a magnetic activated sludge (MAS) process. Bioresour Technol 101:4349–4353

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Haniyeh Jalayeri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jalayeri, H., Doulati Ardejani, F., Marandi, R. et al. Biodegradation of phenol from a synthetic aqueous system using acclimatized activated sludge. Arab J Geosci 6, 3847–3852 (2013). https://doi.org/10.1007/s12517-012-0643-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12517-012-0643-1

Keywords

Navigation